Phylogenetic relationships of the glycoprotein gene of bovine ephemeral fever virus isolated from mainland China, Taiwan, Japan, Turkey, Israel and Australia

Zheng F,Qiu C.

Virol J.

doi:10.1186/1743-422X-9-268

Abstract BACKGROUND:

Theglycoprotein(G)genesequences ofbovineephemeralfevervirus(BEFV) strains derived frommainlandChinahave not been compared with those of the isolates from other countries or areas. Therefore, the G genes of four BEFV isolates obtained frommainlandChinawere amplified and sequenced. Aphylogenetictree was constructed in order to compare and analyze the geneticrelationshipsof the BEFV isolates derived frommainlandChinaand different countries and areas.

RESULTS:

The complete BEFV Ggenewas successfully amplified and sequenced from four isolates that originated frommainlandChina. A total of fifty-one BEFV strains were analyzed based on the Ggenesequence and were found to be highly conserved. Aphylogenetictree showed that the isolates were grouped into three distinct lineages depending on their source of origin. The antigenic sites of G1, G2 and G3 are conserved among the isolates, except for several substitutions in a few strains.

CONCLUSIONS:

Thephylogeneticrelationshipsof the BEFV isolates that originated frommainlandChina,Taiwan,Japan,Turkey,IsraelandAustraliawere closely related to their source of origin, while the antigenic sites G1, G2 and G3 are conserved among the BEFV isolates used in this work.